Publications | XianJun Zhang

Google Scholar

https://scholar.google.com/citations?user=trcMDAsAAAAJ&hl=zh-CN

Research Papers

8. Acquisition of Time-Wavelength-Space Resolved Fluorescence at Low Temperatures in Photosynthetic Cells during Light-Harvesting Regulation

Yuki Fujita, Xianjun Zhang, Shen Ye, Yutaka Shibata*

Under revision. 2024.

7. Access to the Antenna System of Photosystem I Via Single-Molecule Excitation-Emission Spectroscopy

Xianjun Zhang*, Rin Taniguchi, Ryo Nagao, Tatsuya Tomo, Takumi Noguchi, Shen Ye, Yutaka Shibata*

The Journal of Physical Chemistry B. 2024.

6. Optical Spectroscopic Microscopy Targeted to Oxygenic Photosynthetic Membranes and Organisms

XianJun Zhang, Yutaka Shibata*,Shigeichi Kumazaki*

Journal of PhotoChemistry & Photobiology C: Photochemistry Reviews. 2023

5. Accumulation of Quenched LHCII around PSI in Chlamydomonas Cells in State2 Revealed by Cryo-Fluorescence Lifetime Imaging

Yuki Fujita, XianJun Zhang, Ahmed Mohamed Ali, Shen Ye, Jun Minagawa, Yutaka Shibata*

Journal of PhotoChemistry & Photobiology B: Biology. 2022.

4. State Transition Is Quiet Around Pyrenoid and LHCII Phosphorylation Is not Essential for Thylakoid Deformation in Chlamydomonas 137c

XianJun Zhang, Yuki Fujita, Naoya Kaneda, Ryutaro Tokutsu, Shen Ye, Jun Minagawa, Yutaka Shibata*

Proceedings of the National Academy of Sciences (PNAS). (Cover Article). 2022.

3. Chlamydomonas State Transition Is Quiet Around Pyrenoid and Independent from Thylakoid Deformation

XianJun Zhang, Yuki Fujita, Naoya Kaneda, Ryutaro Tokutsu, Shen Ye, Jun Minagawa, Yutaka Shibata*.

BioRxiv. 2021.

2. High-Speed Excitation-Spectral Microscopy Uncovers In Situ Rearrangement of Light-Harvesting Apparatus in Chlamydomonas during State Transitions at Submicron Precision

XianJun Zhang, Yuki Fujita, Ryutaro Tokutsu, Jun Minagawa, Shen Ye, Yutaka Shibata *

Plant & Cell Physiology (Cover Article). 2021

1. Development of a Multicolor Line-focus Microscope for Rapid Acquisitions of Excitation Spectra

Sanker Jana, Yutaka Shibata*

Biophysical Journal. 2020.

Cover Image

It is widely known that photosynthetic organisms redistribute excessive energy through state transition (ST) mechanism by the movement of light harvesting complexes II (LHCII). However, in situ visualizations of the ST in the single-cell level under room temperature remain limited. Using newly developed noninvasive excitation spectral imaging, Zhang et al observed unicellular green algae called Chlamydomonas reinhardtii under ST induction. The home-built excitation spectral microscope provided powerful spectral information about excitation-energy transfer between Chl-a and Chl-b. The technique is sensitive to the Chl-b spectral component specifically bound to LHCII at a small spatial scale. Zhang et al discovered an ST-driven reversible rearrangement of Chl fluorescence.

The cover image shows reconstructed Whole, Chl-b, Chl-a, and Chl-b/Whole ratio images by using excitation spectra during ST induction. A live Chlamydomonas cell is inducted to state 1 (Bottom plane), state 2 (Middle plane), and again state 1 (Top plane) by using illumination with different colors. Red and the light blue arrow are indicated as 710 and 469 nm light respectively.

doi.org/10.1093/pcp/pcab047

Cover Image

Pictured is a section of the optical setup of a high-speed excitation-spectral microscope (ESM). XianJun Zhang et al. used an ESM and high-resolution 3D microscopy to characterize state transition, a regulatory mechanism used by photosynthetic organisms, in the thylakoid membrane of wild-type and mutant strains of Chlamydomonas. The authors found that the thylakoid membrane undergoes fine-scale structural deformations during photosynthesis that were partially irreversible and showed site-dependent differences in state transition efficiency. The results provide insight into the molecular mechanisms underlying photosynthesis in green algae.

doi/10.1073/pnas.2122032119

Oral and Poster

17. 「Study of Energy Regulation within Photosynthetic Organisms via Single-Cell/Molecule Spectroscopy」, The 61th Annual Meeting of the Biophysical Society of Japan, Nagoya, poster, 2023/11/14-16

16. 「Study of Energy Regulation within Photosynthetic Organisms via Single-Cell/Molecule Spectroscopy」, 第八届中国植物蛋白质研究大会, GuiYang, poster, 2023/07/28-08/01

15.「Application of Advanced Microscopic Spectroscopies: Single-Cell/Molecule Analysis」, Prof. ShengBo He Lab, South China Agricultural University, Invited presentation, 2023/03/03

14.「Site-Dependent State Transition and UItrastructural Changes of Thylakoid in Chlamydomonas」, International Symposium on Photosynthesis and Chloroplast Regulation, 日本神戸市, Oral presentation, 2022/11/15-18

13. 「Micro-Spectroscopic Study for Photosynthetic Membrane and Protein-Pigment Supercomplexes」, Minagawa Lab, 日本国立基礎生物研究所 (NIBB) , Invited presentation, 2022/8/29

12. 「Observation of Photosystem I Using Single-Molecule Excitation-Emission Spectroscopy」,The 7th FRIS/DIARE Joint Workshop, 東北大学 , Poster, 2022/8/1

11. 「Site-Dependent State Transition and Ultrastructural Changes of Thylakoid in Chlamydomonas」, International Congress on Photosynthesis Research 2022, New Zealand, Poster, 2022/8/1

10. 「Observation of Photosystem I Using Single-Molecule Excitation-Emission Spectroscopy」, 第29回日本光合成セミナー, On line, 2022/6/25

9. 「Simultaneous Acquisition of Cryo-Excitation-Emission Spectra of Single Photosystem I」, 2021年度生物物理学会　北海道支部-東北支部合同例会，Zoom （On Line）, Oral，2022/3

8. 「Irregular Transformation of Thylakoid upon State Transition as Revealed by Excitation Spectral and Super-Resolution Microcopy」，第59回日本生物物理年会, Tohoku University (On Line), Oral, 2021/11/26

7. 「Excitation-spectral microscopy uncovers in-situ rearrangement of LHCII in Chlamydomonas during state transition 」, 第28回光合成セミナー Zoom (On Line), Oral, 2021/6/28

6. 「Excitation-spectral microscopy uncovers in-situ rearrangement of LHCII in Chlamydomonas during state transition 」, 第11回日本光合作用年会, Zoom (On Line), Oral, 2021/5/28

5. 「Shape-memory behavior of thylakoid membrane in Chlamydomonas as revealed by novel excitation spectral microscopy」，第58回生物物理学会年会，群馬大学 (On Line)，2020/9

4. 「Evaluation of The Movement of Photosynthetic Antenna Complexes in Algal Cells by Excitation Spectral Measurements on Microscope」, International Conference on Artificial Photosynthesis-2019 (ICARP2019), 広島市, 2019/11

3. 「Evaluation of the movement of photosynthetic antenna complexes in algal cells by excitation spectral measurements on microscope」，Summer School, 分子科学学会，京都大学, 2019/8

2. 「Evaluation of the movement of photosynthetic antenna complexes in algal cells by excitation spectral measurements on microscope」，第12回東北大学化学系サマースクール，東北大学，2019/8

1. 「Effective visualization of the excitation　spectra of Chlamydomonas reinhardtii　in vivo by the developed microscope」, 第27回光合成セミナー，大阪大学，2019/7